Research expertise

 

 

Cell culture

Neuronal cultures (Primary cortical neurons, hippocampal-derived immortalised HT-22 cells

differentiation of human dopaminergic neurons)

Microglial cultures (Primary microglia, Immortalised microglial culture)

Astrocyte cutures (Primary astrocytes)

 

In vitro models of cell death (e.g. apoptosis, necrosis, necroptosis, ferroptosis)

 

Cell morphology alterations

Real-time xCELLigence cell impedance

Immunostainings

 

Biochemical analysis

Western Blot, RT-PCR, ELISA

 

Mitochondrial morphology and functional analysis

Confocal microscopy

Mitochondrial isolation/purification

Mitochondrial functional parameters (e.g. Mitochondrial superoxide production, Mitochondrial membrane potential, Oxygen consumption determination (Seahorse/Oroboros technology)

 

Microfluidics

Neurons-on-a-chip

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